Journal: medRxiv

Article Title: Polymorphism in IFNAR contributes to glucocorticoid response and outcome in ARDS and COVID-19

doi: 10.1101/2022.03.10.22272123

Figure Lengend Snippet: (A ) STAT1 expression in the lung after 4-day culture in the presence of IFN beta with or without hydrocortisone (HC). ( B) pSTAT1 expression in the same specimens as in A. ( C) Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. Arrows. ( D ) Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. Ns, not significant; *P<0.05; **P<0.01; and ***P<0.001

Article Snippet: The first stage antibodies were anti-alpha chain of the IFN alpha/beta receptor (St John’s Laboratory STJ112765) that was used 1:2000 and 1:5000 and anti-Stat1 (1:400, 9175S), anti-pStat1(1:100, 9167S) and anti-Stat2 (1:200, 72604S) all from Cell Signalling.

Techniques: Expressing, Translocation Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Anlotinib Benefits the α PDL1 Immunotherapy by Activating ROS/JNK/AP-1 Pathway to Upregulate PDL1 Expression in Colorectal Cancer

doi: 10.1155/2022/8965903

Figure Lengend Snippet: Anlo increased the expression of PDL1 by ROS/JNK/AP-1 pathway in vitro . (a) ROS production was determined and analyzed by flow cytometry. (b) Representative western blots of HSP90, T-JNK, P-JNK, P-C-FOS, P-C-JUN, IFN- α , IFN- β , and IFN- γ . (c) The expression of mRNA levels of IFN- α , IFN- β , and IFN- γ . (d) RNA sequence data analysis of CT26 tumor.

Article Snippet: After blocking with skim milk for 1 hour in TBST, the membranes were incubated with the primary antibodies included anti-PDL1 (#1-76769, Novus, USA, 1 : 1000), anti-PDL1 (#DF6526, affinity, 1 : 1000), anti-HSP90 (#4877, CST, Boston, MA, USA, 1 : 1000), anti-IFN α (#DF6086, affinity, 1 : 1000), anti-IFN β (#ab218229, Abcam, 1 : 1000), Anti-IFN γ (#DF6045, affinity, 1 : 1000), anti-JNK2 (#9258, CST, 1 : 1000), P-JNK (#4668, CST, 1 : 1000), P-c-fos (#5348, CST, 1 : 1000), and P-c-jun (#2361, CST, 1 : 1000) at 4°C overnight.

Techniques: Expressing, In Vitro, Flow Cytometry, Western Blot, Sequencing